Analysis Note

ControlHuman IgG, Mouse IgG, Rat IgG whole molecule, Chicken IgM whole molecule, Goat IgG whole molecule, Guinea Pig IgG, Hamster IgG whole molecule, Rabbit IgG, Rabbit IgG (Fc), and Rabbit IgM whole molecule

Application

Research Sub CategoryIsotype Control Antibodies

Research CategorySecondary & Control Antibodies

Sheep-anti-Rabbit IgG secondary antibody detects level of Rabbit IgG secondary & has been published & validated for use in ELISA.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Immunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fcg receptors which interact with sialylated Fc fragments on IgG molecules.

Immunogen

Rabbit IgG

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Format: Purified

Octanoic Acid and Ammonium Sulfate cut. Depleted against Goat IgG and Rabbit IgM

Purified sheep polyclonal in buffer containing PBS with 0.05% sodium azide.

Quality

Evaluated by ELISA against Human IgG, Mouse IgG, Rat IgG, Chicken IgM, Goat IgG, Guinea Pig IgG, Hamster IgG, Rabbit IgG, Rabbit IgG (Fc), and Rabbit IgM.

ELISA Analysis: A representative lot detected Sheep Anti-Human IgM, (Fc) in ELISA. (1:200 - 1,000 recommended dilution.)

Specificity

This antibody reacts with rabbit IgG and rabbit IgG (Fc) as demonstrated by ELISA. Minimal cross-reactivity detected with Human IgG and Rabbit IgM; little to no cross-reactivity detected with Mouse IgG, Rat IgG, Chicken IgY, Guinea Pig IgG, and Hamster IgG.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

150 kDa calculated